Miseq of Teleogryllus oceanicus gut bacteria: lab female fed artifici... - SRA

SRX2786043: Miseq of Teleogryllus oceanicus gut bacteria: lab female fed artificial diet sample 5
1 ILLUMINA (Illumina MiSeq) run: 19,691 spots, 5M bases, 1.8Mb downloads

Design: 16S rRNA amplicons were generated in a single amplification step using primers 515F (5- GTGCCAGCMGCCGCGGTAA -3) and R806 (5- GGACTACHVGGGTWTCTAAT -3). The amplicon library was prepared by pooling PCR products into equimolar ratios based on qPCR and quantification using a Labchip GX Touch HT (Perkin Elmer). Amplicons in the library were size-selected using a Pippin Prep (Sage Science) and purified using the Qiaquick PCR Purification Kit (Qiagen). The volume of purified library added for sequencing was determined using a Labchip GX Touch HT (Perkin Elmer), and sequenced (uni-directionally) using a 300 cycle MiSeq v2 Reagent Kit and standard flow cell on an Illumina MiSeq platform.

Submitted by: University of Western Australia

Study: Teleogryllus oceanicus Raw sequence reads

PRJNA384097 • SRP106500 • All experiments • All runs

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Examination of gut bacterial communities of wild caught field crickets and lab-reared crickets fed either cat chow or chemically defined artificial diets

Sample: Artificial_diet_female_sample_5

SAMN06887803 • SRS2168072 • All experiments • All runs

Organism: Teleogryllus oceanicus

Library:

Name: ADF23

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: SINGLE

Runs: 1 run, 19,691 spots, 5M bases, 1.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR5512070	19,691	5M	1.8Mb	2017-10-18

ID:: 4016380

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